Part:BBa_K1722003:Design
AckRS can produce a aminoacyl tRNA synthetase.
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 1261
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 450
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The AckRS that we achieved from Shenzhen Second People's Hospital was carried by psi-Check2, which was Amp resistence. After sequencing, we found AckRS has two EcoR1 enzyme cutting site, which are the same as the site in the flank of pSB1C3. So we mutate one of the base pairs on each of the two sites. The plasmid with AckRS that we submitted is EcoR1 free.
Source
We achieved this part from Shenzhen Second People's Hospital, which is Shenzhen University First Hospital. They were doing reseach on unnatural amino acid orthogonal system and fortunately, they agree to provide us the gene.
References
[1] Hausmann C D, Ibba M. Aminoacyl- tRNA synthetase complexes: molecular multitasking revealed. FEMS Microbiol Rev, 2008, 32(4): 705 ~ 721
[2] Han JM, Kim J Y, Kim S. Molecular network and functional implications of macromolecular tRNA synthetase complex. Biochem Biophys Res Commun, 2003, 303(4): 985~ 993
[3] Veronika F, Milan V, Sabine S. Structural basis for the site-specific Incorporation of Lysine Derivatives into Proteins. Plos One, 2014, 9(4): 1~7